摘要
[目的]纯化猪血免疫球蛋白IgG,并测定其轻链与重链的分子量。[方法]采用分步硫酸铵盐析法粗提猪血浆中的免疫球蛋白G,透析袋脱盐后用DEAE-SephadexA50对IgG进行纯化,对纯化后的免疫球蛋白进行SDS-PAGE电泳,采用AlphaEaseFC凝胶分析软件对凝胶图像进行分析。[结果]3份样品中免疫球蛋白的浓度分别为2.712、2.679、2.489 mg/ml;电泳后每泳道均出现2条蛋白带,说明所提免疫球蛋白的纯度较高;2条蛋白带分别为IgG的重链(H链)与轻链(L链),与标准蛋白Marker进行比较可知,H链的分子量约为57 KDa,L链的分子量约为26 KDa。[结论]得到了高纯度的猪IgG,并进一步确定了IgG轻链与重链的分子量。
[Objective] The study was to purify the immunoglobulin IgG from pig blood and determine the molecular weight of its light and heavy chains. [ Method] Immunoglobulin G from pig plasma was extracted by sequential ammonium sulfate precipitation method, and the IgG was purified by DEAE-SephadexA50 after desalination treatment with dialysis bag, the purified immunoglobulin was taken for SDS-PAGE electrophorosis, and the gel image was analyzed with AlphaEaseFC analysis software. [ Result] Immunoglobulin eoncns, of 3 samples were 2. 712, 2.679, 2. 489 mg/ml resp. 2 protein bands appeared in each lane after electrophoresis, which indicated that the purity of the extracted immunoglobulin was higher. The 2 protein bands were the light chain （L chain） and the heavy chain （H chain） of IgG. Compared with standard protein Marker, the molecular weight of H chain and L chain were 57 , 26 KDa resp. [ Conclusion] The study obtained pig IgG with high purity, and determined the molecular weight of its light and heavy chains.
出处
《安徽农业科学》
CAS
北大核心
2009年第23期11017-11018,11039,共3页
Journal of Anhui Agricultural Sciences
关键词
猪血浆
免疫球蛋白
分子量
Pig plasma
Immunoglobulin
Molecular weight
