摘要
比较3种提取猪血清中免疫球蛋白IgG方法的提取效果。分别用辛酸沉淀法,硫酸铵分步沉淀法,辛酸-硫酸铵沉淀法粗提猪血清免疫球蛋白IgG,并通过SDS-PAGE凝胶电泳、AlphaEaseFC凝胶成像分析软件、Bradford蛋白浓度测定法等比较3种方法的提取纯度和得率,同时比较3种方法的时效性和经济性。辛酸沉淀法提取IgG所用时间最短,只需1h,得率较高但纯度不高;硫酸铵盐析法提取的IgG纯度较高、得率也高,但时间较长;辛酸-硫酸铵分步沉淀法所得IgG纯度很高,但所用时间较长,得率也较低。3种提取方法各有特点,可根据不同的实验条件和目的选择不同的提取方法。
Compare three different methods of purifying Immunoglobulin G from swine serum with their purifying effect. Octanoic acid precipitation method,ammonium sulfate by-step precipitation method and octanoic acid-ammonium sulfate coprecipitation method are used to purify IgG from swine serum respectively. SDS-PAGE, Bradford kit and AlphaEaseFC software are used to monitor the purity and yield of extract from serum. In the process of purifying IgG, the octanoic acid precipitation method needs shortest time, only 1 hour. But the purity of extract is lowest ,thus the yield is high. The purity and yield of extract by ammonium sulfate by-step precipitation method are higher, but the time is longer. Meanwhile, the octanoic acidammonium sulfate coprecipitation method gains the purest IgG, but the time is longest and the yield is lowest. The three kinds of methods have their own features individually, they can be adopted in different condition and need.
出处
《中国农学通报》
CSCD
2007年第11期38-43,共6页
Chinese Agricultural Science Bulletin
基金
安徽省教育厅自然科学基金项目"对皖北近年夏季流行猪持续性高热传染病病原体分离鉴定与防治研究"(2004kj307)
国家自然基金项目"恒定链的点突变对融合基因的表达
定位及其免疫应答的影响"(30671537)资助
