摘要
建立了食品中碱性橙、碱性嫩黄O和碱性桃红T染料含量的高效液相色谱检测方法。样品经碱化甲醇提取,提取液在碱性条件下用二氯甲烷萃取,浓缩后用酸化甲醇溶解并用甲醇饱和正己烷萃取净化后经高效液相色谱分离、检测。本方法检测限分别为碱性橙0.02mg/kg、碱性嫩黄O0.03mg/kg、碱性桃红T0.004mg/kg。6种食品样品加标回收率实验所得回收率为72.3%～96.5%,相对标准偏差(RSD)为0.3%～8.8%(n=6),三种染料在0.05～2.5μg/ml浓度范围内均呈良好的线性关系,线性回归系数(r)均大于0.9998。
A method for the simultaneous determination of chrysoidine, auramine O and safranine T in food was developed by high performance liquid chromatography （HPLC）. Samples were extracted using ammonia-methanol （1：50, V/V）. Then the analytes were extracted from the extracts using CH2Cl2, concentrated and redissolved in formic acid- methanol （1：50, WV）, and cleaned up using n-hexane solution saturated with methanol prior to HPLC determination. The linear ranges of this method for determining the three pigments were 0.05- 2.5 μg/ml and the correlation coefficients （r） all were higher than 0.9998. The recoveries for them in 6 kinds of foods spiked at two levels respectively ranged from 72.3% to 96.5%, and the RSDs were 0.3%-8.8% （n = 6）. The limits of detection were about 0.02 mg/kg for chrysoidine, 0.03 mg/kg for auramine O and 0.004 mg/kg for safrauine T.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第14期194-196,共3页
Food Science
基金
国家质量监督检验检疫总局自筹经费项目(2007QK160
2007QK161)
关键词
高效液相色谱法
食品
碱性橙
碱性嫩黄O
碱性桃红T
high performance liquid chromatography
food： chrysoidine
auramine O
safranine T
