摘要
目的：建立一种反相高效液相色谱法测定豆制品中碱性橙。方法：以甲醇＋0.1%甲酸（60：40,V/V）为流动相,在454 nm处测定碱性橙。结果：在最佳实验条件下,色谱峰面积对碱性橙浓度的线性回归方程为A=1.774＋2.9057（r=0.9999）,线性范围为0.02～45μg/ml,检出限为0.008μg/ml;RSD为1.46%～2.28%（n=6）;样品加标回收率为98.0%～100.8%。结论：方法快速、灵敏、重现性好,用于豆制中碱性橙的测定,结果准确。
Objective：A novel quantitative method for the determination of chrisodine in bean products on the reversed-phase high performance liquid chromatography has been developed.Methods：Methanol-0.1% formic acid（60：40,V/V） was used as the mobile phase with λ=454 nm.Results：Under the optimal experimented conditions,the regression equation was A=1.774＋2.9057,the linear range of chrisodine was 0.02～45 μg/ml with r=0.9999 and LOD=0.008 μg/ml.The average recoveries were 98.0%～100.8% with RSD=1.46%～2.28%（n=6）.Conclusion：The method is easy,fast,sensitive,reproducible and practical for the analysis of chrisodine in bean products.
出处
《中国卫生检验杂志》
CAS
2009年第3期545-546,602,共3页
Chinese Journal of Health Laboratory Technology
关键词
碱性橙
反相高效液相色谱法
豆制品
Chrisodine
Reversed- phase high performance liquid chromatograph
Bean products
