摘要
通过15种提取液对辣椒粉中3种碱性橙染料即碱性橙2(CDG)、碱性橙21(AOG)、碱性橙22(AOR)提取效果的比较,建立了基于高效液相色谱法(HPLC)的前处理方法。以体积分数1%甲酸甲醇为辣椒粉中碱性橙提取液,以甲醇和20mmol/L乙酸铵(57:43)流动相,STPAKC18-ES(4.6mm×250mm,5μm)色谱柱,CDG检测波长419nm,AOG、AOR检测波长485nm,流速1.0mL/min,柱温40℃为HPLC条件,该方法的检出限为CDG、AOR:0.01mg/kg,AOG:0.005mg/kg。方法回收率60%以上,相对标准偏差小于4%。结果表明,以体积分数1%甲酸甲醇为提取液时,在阳性样品提取率、阴性样品加标回收以及色谱峰形等方面,与现有国家标准相比,均具有显著优势。
A better extracted method based on high performance liquid chromatography （HPLC） was established for the extraction of 3 alkali orange dyestuffs, namely chrysoidine G （CDG）, astrazon orange G （AOG） and astrazon orange R （AOR） in chili powder by the comparison of the effect of 15 extracted liquids. The sample was extracted with 1% （q）） formic acid methanol, the HPLC separation was carried out on ST PAK C18-ES column （4.6 mmx250 mm, 5 IJm） using methanol and 20 mmol/L ammonium acetate（57：43） as mobile phase under the detection wavelength of CDG 419 nm, AOG and AOR 485 nm respectively, when the flow rate was 1.0 mL/min and column temperature 40 ~C. The limit of quantification of CDG, AOG was 0.01 mg/kg and AOR was 0.005 mg/kg, which was lower than the standard method. Method recovery was more than 60% with the relative standard deviations （RSDs） less than 4%. The result showed that it was superior to national standard method in extraction rate of positive sample, recovery of negative sample and modification of Chromatographic peaks.
出处
《食品科技》
CAS
北大核心
2012年第8期297-300,共4页
Food Science and Technology
基金
浙江省质量技术监督局重大项目(20100104)
关键词
提取液
高效液相色谱法(HPLC)
碱性橙染料
辣椒粉
extracted liquid
high performance liquid chromatography （HPLC）
alkali orange dyestuffs
chili powder
