摘要
[目的]观察苦瓜蛋白对K562/A02细胞多药耐药的逆转及对P-gp表达、功能的影响。[方法]采用CCK-8法测IC50,用流式细胞仪测定苦瓜蛋白对K562/A02细胞上P-gp表达及细胞内ADM潴留的影响。[结果]苦瓜蛋白能提高K562/A02对多种化疗药物敏感性,使P-gp蛋白表达下降,提高细胞内ADM的浓度。[结论]苦瓜蛋白能部分逆转K562/A02的耐药性,逆转机制与下调P-gp蛋白的表达有关。
[Objective] To explore effect of momordin on reversion of multidrug resistance （MDR） in K562/A02 cells, and expres sion and function of P glycoprotein （P-gp）. [Methods] IC50 was detected by CCK-8, and expression of P-gp and retention of ADM in K562/A02 cells were detected by flow cytometry （FCM）. [Results] Momordin can improve sensitivity of K562/A02 cells to many kinds of chemotherapeutical drugs, result in decrease of expression of P-gp, and raise concentration of ADM within cells. [Conclusion] Momordin can partly reverse drug resistance of K562/A02 cells, the mechanism of reversion is related with down regulation of expression of P-gp.
出处
《浙江中医药大学学报》
CAS
2009年第4期475-477,共3页
Journal of Zhejiang Chinese Medical University
基金
浙江省中医药青年基金项目(2006Y013)~~
