摘要
目的探究3-Br PA对肺癌A549/DDP细胞耐药的逆转作用及其机制。方法 1细胞培养:RPMI1640培养基培养肺癌A549细胞及人肺腺癌耐顺铂细胞株A549/DDP细胞;2采用CCK8及Western blot法观察3-Br PA对A549/DDP细胞的耐药逆转作用和P-糖蛋白(P-glycoprote,P-gp)的表达水平;采用流式细胞术检测不同浓度3-Br PA作用A549/DDP细胞后细胞内罗丹明-123(Rhodamine-123,Rh123)的蓄积情况。结果顺铂对A549细胞、A549/DDP细胞及3-Br PA作用后的A549/DDP细胞的IC50分别为6.714μg/ml、38.99μg/ml及12.86μg/ml,逆转倍数为3.03,相对逆转效率为81%。3-Br PA可使A549/DDP细胞的P-gp表达下调。3-Br PA可使A549/DDP细胞内的Rh123蓄积增多并呈剂量依赖性。结论 3-Br PA可逆转A549/DDP细胞对顺铂耐药,作用机制可能与其抑制P-gp的功能和表达有关。
Objective To preliminarily explore the reversal effect of 3-Br PA on multidrug resistance of lung cancer cell line A549/DDP and to study its potential mechanism. Methods ①Cell culture： The human lung adenocarcinoma cell line A549 and cisplatin-resistant cell line A549/DDP cells were grown in RPMI1640 medium; ②CCK8 and Western blot were used to observe the reversal effect of 3-Br PA on cisplatin-resistant cell line A549/DDP cells and the expression of P-gp. Flow cytometric technology was used to detect different concentrations of 3-Br PA on A549/DDP cells by measuring Rh123 mean fluorescence intensity（ MFI）. Results The IC50 of cisplatin to A549 cells,A549/DDP cells and A549/DDP cells after 3-Br PA added were 6.714 μg/ml,38.99 μg/ml and 20.2 μg/ml,respectively. The reversal ratio was 3.03 and the relative reversal efficiency was 81% as compared to A549 cells; The 3-Br PA can make the expression of P-gp on A549/DDP cells dropped. The result of FCM shows that 3-Br PA can increase the accumulation of Rh123 on A549/DDP cells in a dose-dependent manner. Conclusions 3-Br PA can reverse the drug resistance on A549/DDP cell line to cisplatinum in a certain extent,which may be attributed to down regulating of P-gp expression.
出处
《中华肺部疾病杂志（电子版）》
CAS
2016年第2期145-149,共5页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
国家自然科学基金资助项目(81372340)
关键词
支气管肺癌
3-BrPA
A549/DDP细胞
耐药逆转
Bronchus lung cancer
3-BrPA
Cisplatin-resistant cell line A549/DDP cells
Resistance reversal
