摘要
目的观察苦瓜蛋白诱导K562细胞凋亡时细胞粘附分子(CD54、CD44)表达水平的变化,探讨粘附分子在苦瓜蛋白诱导K562细胞凋亡中的作用。方法以一定浓度的苦瓜蛋白处理K562细胞;CCK-8试剂盒检测其对细胞生长的影响;流式细细术(An-nexinⅤ)、透射电镜等方法检测细胞凋亡;同时应用流式细细术检测粘附分子(CD54、CD44)蛋白表达水平的变化。结果苦瓜蛋白对K562细胞生长具有明显的抑制作用;流式细胞术及形态学观察(电镜)证实一定作用浓度的苦瓜蛋白可诱导K562细胞发生明显的细胞凋亡;苦瓜蛋白处理组K562细胞中CD54、CD44表达分别为18.62%和1.32%,对照组分别为0.25%和0.17%,分别上调了18.37%、1.15%。结论苦瓜蛋白引起K562细胞凋亡的过程中,粘附分子CD54表达上调;CD54表达上调可能是苦瓜蛋白诱导肿瘤细胞凋亡的机制之一,其机制可能涉及细胞粘附依赖性细胞凋亡。
AIM： To explore the change of the expression of cell adhesion molecule CD54 and CD44 in erythroleukemic K562 apoptosis cells induced by momordin from momordica charantia seeds and study the effects of cell adhesion molecule CD54 and CD44 on cell apoptosis induced by momordin. METHODS： After the treatment of K562 cells with appropriated concentration momordin, CCK-8 test was employed to determine K562 ceils growth; flow cytometry FACScan （FITC-Annexin V staining） and electron microscopy were used to detect apoptosis; The expression of CD54 and CD44 were examined by flow cytometry FACScan （FITC-CD54 and PE-CD44 staining）. RESULTS： CCK-8 test showed K562 cells growth was significant inhibited by momordin; the apoptosis was detected by cell morphology and flow cytometry FACScan （FITC-Annexin Ⅴ） in K562 cells after treatment by appropriated concentration momordin. The expressions of CD54 and CD44 in momordin treated K562 cells were 18.62% and 1.32% respectively, and in negative momordin treated K562 cells were 0.25% and 0.17% respectively, and momordin could up-expresses the protein of CD54 18.37% and CD44 1.15%. CONCLUSION： Momordin can markedly induce the K562 cell to apoptosis. The up-expressions of CD54 exist in the process of apoptosis induced by momordin. The change of cell adhesion molecule maybe one of the key factors in the mechanisms of apoptosis induced by momordin, and its mechanism maybe involve in adhesion-dependent apoptosis.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第12期1403-1407,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
