摘要
以3，5-二硝基苯甲酰氯为衍生化试剂，柱前衍生，ODS2柱分离，V（甲醇）：V（0．01mol／LKH2P04）=60：40（H3PO4调节pH3．5）洗脱，紫外（230nm）检测，建立了黄芪甲苷的反相高效液相色谱法。在5．0～100μg/mL范围内，黄芪甲苷的峰面积与其浓度呈现良好的线性关系。回归方程为A=478944＋2132c，相关系数为0．9986。相对标准偏差为2．7％（c=20．0μg/mL，n=6），加标回收率在97．8％～103．4％。该法已用于黄芪中黄芪甲苷含量的测定。
A pre-column derivatization procedure is described for the detennination of astragaloside Ⅳ in radix astragali by HPLC. 3,5-dinitrobenzoyl chloride was used for the defivatization of astragaloside Ⅳ. The separation of astragaloside 1V derivatives was accomplished on a column packed with 5 tan ODS2 （octadecylsilance）. The mobile phase of CH3OH-KH2PO4（0.01 mol/L, adjusted pH 3.5 by H3P04） =60：40 （V/V） was used throughout. The experimental results were detected at 230 nm. The results show that a good linear relationship exists between the peak area and the concentration in the range of 5.0- 100μg/mL for astragaloside IV. The regression equation is A = 478944 ＋ 2132c, and the correlation coefficient is 0. 9986. The relative standard deviation is 2.7 % （ c = 20.0 μg/mL, n = 6）. The recoveries are in the range of 97.8% - 103.4%. The method has been applied to the determination of astragaloside Ⅳ in Radix Astragali with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第7期108-111,共4页
Chinese Journal of Analysis Laboratory
