摘要
【目的】为开展猪带绦虫的病原学形态观察、生物学特性研究以及解决实验材料来源的问题,建立猪带绦虫的实验仓鼠动物模型。【方法】通过经口感染被免疫抑制的实验仓鼠,观察仓鼠体内绦虫的感染、生长发育及寄生部位等生物学特性,并利用压片技术和组织切片技术对检出的绦虫的形态结构进行研究。【结果】免疫抑制剂在5mg/只,感染一次就能使囊尾蚴发育到性成熟;在实验仓鼠体内,感染后40d的仓鼠体内就能检出具有体节的可见虫体,感染80d后的仓鼠体内能检出孕卵节片;检出的虫体多数吸附在小肠前1/3段,也有寄生在胆囊和胆管中的虫体,甚至偶见虫体钻出胆囊外堆积在肝脏附近;虫体头节上可见4个吸盘及两圈小钩;与人猪带绦虫相比,成熟节片较少。【结论】成功建立了猪带绦虫仓鼠动物模型,这使得成功解决猪带绦虫实验材料的来源问题成为可能,也为猪带绦虫的深入研究奠定了基础。
[Objective] To carry out the observation of pathogen, biologic characteristics of Taenia solium and solve the problem of material source for Taenia solium, experimental model of Taenia solium in hamsters was established. [Method] The immune-suppressed hamster was infected orally with Cysticercus cellulosa in order to observe the biological characteristics of Taenia solium in hamsters such as infectivity, growth and development and parasitic site, and the morphological structure of detected cestode in hamsters was obersved using the squash technique and tissue section technique. [Result] At the 5mg dose of the t, the hamster was infected only one time, and Cysticercus cellulosa could grow to adult. At the same time, the detectable rate of Taenia solium was very high, the cestode containing somite could be detected in the hamste, after 80d infection, gravid proglottid could be detected. The most polypide adsorbed in ahead of 1/3 small intestine, some were also found in gall bladder and bile duct, even some escaped form gall bladder and parasited in liver. Four suckers and two cycles of granular structure were found in scolex. Compared with human-taenia solium, mature proglottids were fewer. [Conclusion] The experimental model of Taenia solium in hamsters has been successfully developed in this experiment. This made it becomes possible to successfully solve the problem of material source for Taenia solium, and has also laid a foundation for the further research of Taenia solium.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第6期2172-2177,共6页
Scientia Agricultura Sinica
基金
甘肃省生物技术研究与开发项目(GNSW-2003-27)
关键词
猪带绦虫
仓鼠动物模型
绦虫特性
Taenia solium
hamster animal model
taenia characteristics
