摘要
采用醋酸可的松诱发Wistar大鼠、BALB/C小鼠和家兔感染卡氏肺孢子虫,比较不同动物对卡氏肺孢子虫的易感性。经姬氏染色法、哥氏银染色法等染色后进行虫体形态观察,结果显示,Wistar大鼠卡氏肺孢子虫惑染率最高(100%),虫体量多。不同给药方式对Wistar大鼠卡氏肺孢子虫的感染率无显著性意义。各种染色法可显示肺组织印片或切片的滋养体或包囊。姬氏染色法是显示印片中虫体的可靠方法。改良哥氏银染色法和快速焦油紫法是显示肺组织石蜡切片中包囊的可靠方法。伦氏荧光桃红——肼黄法能良好地显示切片中的滋养体和囊内小体。
Pneumocystis carinii infection was induced in normal Wistar rats, BALB/C mice and rabbits after administration of cortisone acetate and it was found that sensitivity of these animals to infection was different with Wistar rats being the most susceptible. Imprints and sections prepared from infected pulmonary tissue were stained and examined. Five stain techniques including Giemsa's stain, modified Gomori-Grocott's methenamine silver stain etc., were used to demonstrate cysts, trophozoites or intracystic bodies in these imprints or tissue sections. It was found that Giemsa's stain, modified Gomori-Grocott's methenamine silver stain, rapid cresyl violet stain and Lendrum' sphloxine-tartrazine stain were all useful to demonstrate the existence of these pathogens.
出处
《上海第二医科大学学报》
CSCD
1989年第1期7-11,共5页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
卡氏肺孢子虫
肺炎
动物模型
感染
Pneumocystis carinii Pneumocystis carinii pneumonia animal model opportunistic infection
