摘要
采用毛细管区带电泳直立式安培电化学检测方法分离和测定单糖。考察了电解质溶液的ｐＨ和浓度对单糖分离的影响，适量加入氯化钠可改善分离条件。所列单糖的浓度至少在００２～０２ｍｍｏｌ／Ｌ范围内与检测电流峰高呈线性关系。检测限为１５×１０－６ｍｏｌ／Ｌ。应用该法进行了乳糖的组成和人血中葡萄糖的测定。
Capillary zone electrophoresis with vertical amperometric detection was employed for the separation and determination of monosaccharides.The effect of pH and electrolyte concentration on the separation of monosaccharides was investigated.The addition of sodium chloride improves the separation.The detected peak current was found to be linearly proportional to the monosaccharide concentrations in the range of 002～02 mmol·L-1.The detection limit is 15×10-6mol·L-1.The method has been applied to the determination of lactose and glucose in human blood samples.
出处
《分析测试学报》
CAS
CSCD
1998年第3期9-12,共4页
Journal of Instrumental Analysis
关键词
毛细管电泳
单糖
电化学检测
测定
分析
Capillary electrophoresis,Electrochemical detection,Monosaccharide
