摘要
用自制高效毛细管电泳装置研究毛细管电泳电导检测，由用阴离子交换膜管制作的导电接口连接电泳毛细管和电导池，高电压被有效隔离，实现柱后电导检测，用长６０ｃｍ、内径５０μｍ石英毛细管，以ｐＨ４．０的７×１０￣（－３）ｍｏｌ／ＬＮａＡｃ￣（－２）×１０￣（－３）ｍｏｌ／ＬＨＩＢＡ－ＨＡＣ溶液为载体，在１８ｋＶ电压下，轻重三种稀土Ｎｄ、Ｇｄ、Ｙｂ的混合物８ｍｉｎ内完成分离检测。用上述毛细管，以ｐＨ为５．８的５×１０￣（－３）ｍｏｌ／ＬＮａＡｃ－ＨＡＣ溶液为载体，在２０ｋＶ电压下乙二胺、三乙胺、组氨酸、谷氨酸混合有机物７．５ｍｉｎ内完成分离检测。
The conductivity detection for capillary zone electrophoresis was studied with a self-asembled apparatus.A conductivity joint,made up of a tubular anion exchange membrane,was used to connect the electrophorcsis capillary and the conductance cell.Thus the high potential electricity was effectively isolated,and off-column conductivity detection was realized.The separation and detection of rare earth metal ions Nd3+,Gd3+ and Yb3+were achieved using 7×10-3mol/L NaAc-HAc buffor(pH 4. 0)containing HIBA-HAc solution as carrier with a fused silica capillary of 60 cm long and 50μm i.d.at 18 kV voltage in less than 8 min. The separation and detection of ethylenediamine,triethylamine,histidine,and L-glutamic acid were achieved using 5× 10-3mol/L NaHc-HAc(pH 5. 8)as carrier and above mentioned capillary at 20 kV voltage in less than 7.5 min.
出处
《分析测试学报》
CAS
CSCD
1996年第1期37-40,共4页
Journal of Instrumental Analysis
基金
中国有色金属工业总公司和湖南省教育委员会科学基金
关键词
毛细管区带电泳
电导检测
稀土
胺
氨基酸
Capillary zone electrophoresis
Conductivity detection
Rare earth elements
Amine
Aminoacid.
