摘要
利用葡萄糖和果糖在碱性条件下烯醇化反应速度不同，提出了速差动力学光度法同时测定葡萄糖和果糖的新方法。通过实验条件的选择，确定了葡萄糖和果糖与斐林试剂反应，与磷钼酸显色的动力学反应级数为零级；且反应表观速率常数分别为：０．１０８ｍｇ／Ｌ·ｍｉｎ，０．４２７ｍｇ／Ｌ·ｍｉｎ；测量浓度的线性范围均为：２～８ｍｇ／Ｌ；最低检测限为１．０×１０－９ｇ／Ｌ；对人工合成样品测定结果的相对标准偏差为１．２０％～３４１％；对实际样品测定结果的标准回收率为９５％～１０２％。
This paper presents a new method of simultaneous determination of glucose and fructose by differential reaction-rate kinetic spectrophotometry based on the difference of enolization reaction-rate of glucose and fructose in basic medium.Under optimum experiment conditions,the kinetic reaction grades of glucose and fructose reacting with Fehling reagent and phosphomolybdic acid are zero;the apparent constants of reaction-rate of glucose and fructose are 0.108 mg/L·min,0.427 mg/L·min,respectively.The linear determination ranges of glucose and fructose are 2～8 mg/L.The minmum detectability is 1.0×10 -9 g/L.The RSD% for artificial samples is 1.20%～3.41%.The recovery for real samples is 95%～102%.
出处
《南昌大学学报（理科版）》
CAS
1999年第1期81-85,共5页
Journal of Nanchang University(Natural Science)
基金
江西省自然科学基金
关键词
光度法
同时测定
葡萄糖
果糖
测定
differential reaction-rate kinetic sepctrophotometry,simultaneous determination,glucose,fructose
