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MiRNA的电化学检测方法研究 被引量：2

Investigation of electrochemical detection of miRNA
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摘要 MiRNA（microRNA）是一类非编码、内源性的小RNA分子（17～25个碱基），其在生物体内含量较低，通过其与靶基因mRNA结合参与动植物体内多种基因的表达与调控。作为癌基因或抑癌基因，其在肿瘤的发生、发展过程中起着重要作用，因而对miRNA的检测可为癌症的诊断以及临床治疗提供重要依据。该文总结了针对miRNA几种不同的检测方法，重点综述了几种高灵敏的电化学检测方法。miRNA的检测对肿瘤的早期诊断具有重要的生物学意义，对临床上癌症的预警和治疗这一复杂科学问题的研究起到促进作用。 MicroRNAs （miRNAs） are non-coding, endogenous RNA molecules which possess 17 ~ 25 nucleotides. Acting as oncogenes or tumor suppressors, miRNAs play a key role in regulating gene expression and are believed to be important for developing novel therapeutic treatments and clinical prognoses. This review addresses several methods for detection of miRNA, with emphasis placed on highly sensitive electrochemical characterization. The detection of miRNA is of great biological significance for clinical diagnosis as well as cancer treatment.
作者 衣馨瑶 张宇 阳明辉 王建秀
机构地区 中南大学化学化工学院
出处 《化学传感器》 CAS 2012年第4期9-15,共7页 Chemical Sensors
基金 国家自然科学基金(No.21105128) 高等学校博士学科点专项科研基金(20100162110018)
关键词 MIRNA 癌症 电化学检测 miRNA cancer electrochemical detection
分类号 O657.1 [理学—分析化学]
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参考文献51

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同被引文献21

  • 1Houbavity HB, Dennis L, Jaenisch R, et al. Characterization ofa highly variable eutherian microRNA gene[ J ]. RNA, 2005 , 11(8): 1245-1257.
  • 2Zhou H, Rigoutsos I. MiR-103a-3p targets the 5, UTR ofGPRC5A in pancreatic cells [ J ]. RNA, 2014,20(9): 1431-1439.
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化学传感器
2012年 第4期

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