摘要
用自制的高效毛细管电泳－电化学检测装置，以３５０μｍ铜圆盘电极为工作电极，在碱性溶液中，对７种多羟基抗生素的分离进行了研究。结果表明：浓度线性范围可达２至３个数量级，最低检测限为５．０×１０^（－７）ｍｏｌ／Ｌ。在８ｈ内连续测定，峰电流和迁移时间的相对标准偏差分别为７．６％和２．２％。实际样品（丁胺卡那霉素）测定回收率为９９．６％。
Capillary electrophoresis(CE) was employed to separate polyhydroxy antibiotics in alkaline solution, combined with electrochemical detection at copper electrode. The electrode used was a 350 μm diameter Cu disk operated in a wall-jet configuration. A sample mixture containing 7 different polyhydroxy antibibeics was separated within 30 min. The linearity varied over a 2-3 order of magnitude and the detection limits were 0.5 μmol/L.The reproducibility for the peak current and the migration time of 50 μ mol/L doublemycin injected every hour in a period of 8h, was 7.6%and 2.2%RSD, respectively, and the recovery of the determination of the real sample was 99.6%.
出处
《高等学校化学学报》
CSCD
北大核心
1995年第10期1514-1518,共5页
Chemical Journal of Chinese Universities
基金
中国科学院长春应用化学研究所电分析化学开放实验室
关键词
毛细管电泳
电化学检测
多羟基
抗生素
Capillary electrophoresis
Electrochemical detection
Polyhydroxy antibiotics
Copper electrode
