摘要
目的观察黄荆子乙酸乙酯提取物(the acetoacetate extract of Vitexnegundoseed,Evn-50)和顺铂(cisplatin,DDP)在体外对人卵巢癌细胞株COC1凋亡的影响,探讨Evn-50提高COC1细胞株对顺铂化疗敏感性的机制。方法Evn-50,DDP以及两药联合分别处理人卵巢癌COC1细胞株,台盼蓝染色计数法检测药物对细胞生长的抑制率,并用流式细胞术、AO/CB荧光双染色法测Evn-50,DDP以及两药联合对细胞凋亡的影响。结果细胞抑制率在联用组中随Evn-50剂量的增加而增加,且明显高于同剂量单用组(P<0.01);细胞凋亡率在联用组中随Evn-50剂量的增加而增加,且高于同剂量单用组(P<0.01),流式细胞仪分析结果显示Evn-50呈浓度依赖性将COC1细胞阻滞在G2/M期,而DDP将COC1细胞阻滞在G1/S期。结论Evn-50有增强顺铂诱导卵巢癌细胞株COC1凋亡的作用,其机制可能与二者对卵巢癌细胞株COC1周期阻滞点不同有关。
Objective To explore the effects of the acetoacetate extract of Vitex negundo seed （Evn-50） and cisplatin （DDP）on the inducing apoptosis of ovarian carcinoma cell line COC1 in vitro and the potential mechanism of increased response rate of DDP induced by Evn-50. Methods Human ovarian carcinoma cell line,COCl,was respectively treated by Evn-50, DDP and Evn-50 combined with DDP. Growth inhibition was measured by trypan blue exclusion test.The arrest of cell cycle and the influence induce human ovarian carcinoma cell line COC1 apoptosis after they treated by Evn-50,DDP and Evn-50 combined with DDP was detected by PI staining flow cytometry and AO/EB double fluorescence stain. Results In Evn-50＋ DDP group , the inhibitory rate of COC1 cells increased more than Evn-50 group （P〈0.01） or DDP group （P〈0.01）.The effects of growth inhibition were dependent on the concentration of Evn-50. In Evn-50＋DDP group,the index of apoptosis increased more than Evn-50 group （P〈0.01） or DDP group （P〈0.01）.With the increase of Evn-50 concentration,the index of apoptosis increased in Evn-50＋DDP group.Flow cytometry analysis revealed that treating COC1 cells with increasing quantities of Evn- 50 increased the percentage of cells in the G2/M phase,and treating COC1 cells with DDP increased the percentage of cells in the G1/M phase. Conclusion Evn-50 can enhance DDP to induce Human ovarian carcinoma cell line apoptosis,and they are arrest the cells in different phrase.
出处
《中国现代医药杂志》
2009年第5期5-8,共4页
Modern Medicine Journal of China
关键词
黄荆子
顺铂
卵巢癌
凋亡
细胞周期
Vitex negundo seed Cisplatin Ovarian carcinoma Apoptosis Cell cycle
