摘要
目的观察甲基莲心碱(Nef)在体外对长春新碱抑制人胃癌细胞增殖及诱导人胃癌细胞凋亡的影响。方法采用MTT比色法、软琼脂克隆形成法检测药物的细胞毒性作用,并用流式细胞术、AO/EB荧光双染色法测定Nef对长春新碱诱导人胃癌细胞凋亡的影响。结果2.5,5,10μmol/LNef能增强长春新碱对人胃癌细胞(SGC7901)增殖的抑制作用;10μmol/LNef能增强长春新碱(0.1,0.5,2,4μg/ml)诱导SGC7901细胞凋亡。结论甲基莲心碱能增强长春新碱抑制人胃癌细胞增殖及诱导人胃癌细胞凋亡,为一种低毒高效的化疗增敏剂。
To study the effects of nefefine on gastric carcinoma cell proliferation inhibited and induced apoptosis by vincfistine in vitro. Methods Cytotoxicity assay was tested by MTr and soft agar colony forming assay, The influence of nefefine to affect vincfistine to induce gastric carcinoma apoptosis was detected by PI staining flow cytometry and AO/EB double fluorescence stain. Results 2.5, 5, 10μmol/L Nef enhanced vincfistine to inhibit the proliferation of SGC 7901 cells; 10μmol/L Nef enhanced vincfistine （0.1,0.5,2,4ug/ml）to induce SGC7901 cells apoptosis. Conclusion Nef enhanced vincfistine to inhibit the proliferation of gastric carcinoma and induce gastric carcinoma cells apoptosis. It is a kind of low poisonous and high effective chemosensitizera.
出处
《海南医学》
CAS
2008年第6期113-115,共3页
Hainan Medical Journal
关键词
甲基莲心碱
长春新碱
化疗增敏剂
胃癌细胞
增殖
凋亡
Nefefine
vincristine
chemosensitizer, gaatric carcinoma
proliferation
apoptosis
