摘要
目的 探讨RelA反义寡核苷酸对卵巢癌细胞株COC1凋亡的影响。方法 应用不同浓度的肿瘤坏死因子 (TNF)α或泰素结合RelA反义寡核苷酸 ,分别处理COC1细胞株 ;应用间接免疫荧光、Westernblot、流式细胞术、DNA凝胶电泳等方法 ,检测RelA活化及COC1细胞凋亡。结果 5 0nmol/L的泰素单独及其与RelA反义寡核苷酸联合处理COC1细胞 12h后 ,COC1细胞的凋亡率分别为 (12 3± 0 6 ) %、(2 7 4± 0 5 ) % ,两者比较 ,差异有极显著性 (P <0 0 1) ;2 4h后 ,COC1细胞凋亡率分别为 (13 0± 0 5 ) %、(31 7± 0 3) % ,两者比较 ,差异有极显著性 (P <0 0 1)。 5 0 μg/L的TNFα单独及其与RelA反义寡核苷酸联合处理COC1细胞 12h后 ,COC1细胞凋亡率分别为 (13 2± 0 4 3) %、(30 8± 0 3) % ,两者比较 ,差异有极显著性 (P <0 0 1) ;同样 10 0 μg/L及 2 5 0 μg/LTNFα单独及其与RelA反义寡核苷酸联合处理COC1细胞的凋亡率相比 ,差异均有极显著性 (P <0 0 1)。结论 RelA反义寡核苷酸可增加TNFα或泰素诱导卵巢癌细胞凋亡的敏感性。
Objective To evaluate the effects of RelA antisense oligonucleotide on apoptosis of ovarian cancer cells Methods COC1 cell line was treated with RelA antisense oligonucleotide combining withtumor necrosis factor (TNF) α or paclitaxel at appropriate concentrations and duration The apoptosis and RelA activition of ovarian cancer line COC1 cell were measured by indirect immunofluorescence, Werstern blot, flow cytometric analysis, DNA ladder assay Results The apoptosis increased obviously in COC1 rells if treated by RelA antisense oligonucleotide combining with paclitaxel 50 μmol/L than paclitaxel 50 μmol/L only (27 4±0 5)% vs (12 3±0 6)% ( P <0 01), when treated 12 hs; (31 7±0 3)% vs (13 0±0 5)% ( P <0 01) when treated 24 hs. The apoptosis was also increased obviously in COC1 cells if treated by RelA antisense oligonucleotide combining with TNF α 50 μg/L than TNF α 50 μg/L alone. (30 8±0 3)% vs (13 2±0 4)% ( P <0 01).Conclusion RelA antisense oligonucleotide may induce apoptosis susceptibility of COC1 cells to TNF α or paclitaxel
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2002年第4期202-204,共3页
Chinese Journal of Obstetrics and Gynecology
基金
国家自然科学基金资助 (3 9870 765 )
2 0 0 0年国家杰出青年基金资助 (3 0 0 2 5 0 17)
