摘要
目的:探讨"补虚化瘀"针法对骨质疏松大鼠Ⅰ型胶原基因表达的影响。方法:选择8月龄W istar雌性大鼠50只,随机抽取10只作为空白对照组即假手术组,其它40只做去势手术。3个月后将去势大鼠随机分成模型组、针刺组、针刺加雌激素组、雌激素组。共治疗12周。治疗结束后,取大鼠股骨做Ⅰ型胶原mRNA原位杂交检测。结果:实验表明,治疗组与模型组相比,Ⅰ型胶原mRNA在成骨细胞内表达明显,且细胞数目增多。经图像分析系统测定,阳性反映面积与灰度测值均有明显差异(P<0.01)。结论:"补虚化瘀"针法对骨质疏松大鼠有治疗作用,可增强Ⅰ型胶原mRNA表达的活性。
Objective ：To explore the effect of acu--therapy of ＂strength -xu and dissolve -yu＂ on the rats＇ expression of type I collagen mRNA. Methods：50 eight months old wistar rats, there are 40 rats to ovariectomy and the other （sham group） are not. After three months ,ovariectomized rats were trabantly divided into model, acupuncture, acupuncture adding estrogen and estrogen groups. The treatments are continue 12 weeks. After ending treatment ,to detect type I collagen mRNA. Results ： All the femoral bones of rats were detected by hybridization in situ. Treatment groups compare with nlodel group, that type I collagen mRNA expression was obviously in oasesous cell. To deal with image analysis sys- tem, we got the datas of positive staining area and gray scale value. To compare the datas between model group and other groups, they had obviously different （ P 〈 0.05 or P 〈0. 01 ）. The sham group and treatment groups were no diference. （ P 〉 0.05）. Conclusion ：＂ strength - xu and dissolve - yu＂ techniques of needling has effect on rat bone with osteoporosis. It can strength actively express of type I collagen mRNA.
出处
《中华中医药学刊》
CAS
2009年第5期1034-1036,共3页
Chinese Archives of Traditional Chinese Medicine
基金
吉林省教育厅资助项目(吉教2004[35]号)
关键词
骨质疏松
补虚化瘀针法
Ⅰ型胶原mRNA
postmenopausal osteoporosis
＂strength - xu and dissolve - yu＂ techniques of needling
bone density
type Ⅰ collagen mRNA
