摘要
目的探讨己烯雌酚对去卵巢大鼠骨组织形态学及代谢的影响。方法3月龄雌性SD大鼠27只,分对照组、去卵巢未治疗组和去卵巢治疗组3组。去卵巢治疗组在去卵巢术后,给予己烯雌酚0.022 5 mg/d·kg·b.w.灌胃,每周6 d,持续90 d。用图像分析仪观测各组大鼠胫骨近端骨计量学参数,并在扫描电镜下观察大鼠股骨的结构改变。检测血中胆固醇浓度及主要内脏的质量。结果与对照组比较,去卵巢未治疗组大鼠胫骨骨小梁面积减少65%,骨小梁间隙增加189%,骨形成率增加92%;股骨的骨小梁几乎消失,骨组织的三维构筑受到破坏。与去卵巢未治疗组比较,治疗组骨小梁面积增加128%,骨小梁间隙减少63%,骨形成率减少49%;股骨的骨小梁排列整齐,连接紧密。去卵巢治疗组大鼠无血中胆固醇增高及内分泌改变,子宫质量增加。结论己烯雌酚能有效地预防骨质疏松症,但具有导致生殖器官癌变的危险性。
Objective To study the effects of stilbestrol on bone growth and metabolism in ovariectomized rats. Methods Twenty-seven 3-month-old female SD rats were randomly divided into 3 equal groups, namely the control group, ovariectomi- zed group (OEG), and OEG+estrogen (OEG+E) group. After ovariectomy, the rats in the third group were given stilbestrol 0.022 5 mg/d·kg·b.w. by intragastric gavage for 90 d. A digital image analysis system was used for the measurement of histomorphometric parameters in the proximal tibial metaphysis of the rats. The three-dimensional structure of the femur was observed with scanning electron microscope, with the blood cholesterol level determined and the main viscera weighted. Results In comparison with the control rats, the trabecular bone area (TbAr) of ovariectomized rats was significantly reduced (-65%), trabecular bone space (TbSp) enlarged (+189%), and the bone formation rate (BFR) accelerated (+91%). The cancellous bone of the femurs manifested obvious signs of thinning, roughening and resorption lacunae to cause trabecular bone discontinuance, and osteoporosis occurred. The rats treated with stilbestrol, in contrast, had increased TbAr (by 128%), reduced TbSp (by 63%), lowered BFR (by 49%), and reduced osteoclasts (by 32%). The cancellous bone of the femurs was well arranged in close connection with each other, and the high cholesterol level and endocrinic changes in response to ovariectomy was corrected by stilbestrol, which also caused the increase of the uterine weight. Conclusion Stilbestrol may help prevent primary osteoporosis but may increase the risk of female genital cancer.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第8期798-801,共4页
Journal of First Military Medical University
基金
广东省卫生厅科研项目(A2001485)~~
