摘要
研究了香荚兰细胞悬浮培养的生长周期、可溶性糖浓度、ｐＨ值变化及植物生长调节剂对细胞生长与香兰素产生的影响．发现细胞悬浮培养生长周期以１４ｄ为宜，ＮＡＡ比２，４－Ｄ更适合细胞生长及香兰素形成．
The subculture intervals, variations of soluble sugar contents and pH values and effects of plant growth regulators on cell growth and vanillin production were studied during batch cell suspension culture of Vanilla planifolia . It was found that the proper subculture interval was 14 days, NAA could improve the cell growth and biosynthesis of vanillin, and the inhibition of 2,4 D on the formation of vanillin could be alleviated partly by using cytokinins in combination of it.
出处
《华中理工大学学报》
CSCD
北大核心
1998年第5期8-10,共3页
Journal of Huazhong University of Science and Technology
关键词
香荚兰
悬浮培养
香兰素
Vanilla planifolia
suspension culture
vanillin
