摘要
以化学合成并证实抗菌活性和抗菌谱都高于天然抗菌肽的杂合肽ＣｅｃｒｏｐｉｎＡ１－１１Ｄ１２－３７的氨基酸序列为基础，选用酵母高频使用密码子设计了一种新型抗菌肽基因，基因合成采用二次ＰＣＲ（聚合酶链式扩增）方法．设计和合成的基因全长１４０个碱基对，包括氨基酸编码序列、起始密码子、终止密码子和两端限制性内切酶ＢａｍＨＩ、ＥｃｏＲＩ、ＳａｌＩ识别顺序，合成的基因克隆于ＰＣＲＴＭ２．１载体上．经ＤＮＡ序列分析证实，合成基因碱基序列与设计序列完全一致．
A novel cecropin AD gene was designed and synthesized, in which DNA sequence is based on the amino acid sequence of hybrid peptide cecropin A1-11D12-37. The codon selection of the designed gene was carried out according to the coden usage of yeast. The designed gene is 140 bp in length, including the encoded sequence, the start and stop codons, and the restriction sites of BamH I, EcoR I and Sal I at both ends. The cecropin AD gene was synthesized by twice PCR method and cloned into PCRTM 2.1 vector. It was shown that the DNA sequence of the synthetic cecropin AD gene coincides with that of the designed gene by DNA sequencing.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1998年第3期60-63,共4页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省自然科学基金
