摘要
将抗菌肽ＡＤ基因定向克隆到大肠杆菌－酵母穿梭质粒ｐＣＬＷＡ２的ＢａｍＨＩ和ＳａｌＩ位点上，构建成含抗菌肽ＡＤ基因的重组质粒ｐＣＡＤ，转化酵母宿主菌ＡＢ１０３，转化子在缺乏亮氨酸的ＳＤ选择培养中３０℃培养４８ｈ，培养液经简单纯化后，活性蛋白ＰＡＧＥ和琼脂孔穴扩散法测定抑菌活性表明，抗菌肽ＡＤ基因在酵母中获得表达．
The synthetic cecropin AD gene was inserted into the BamH Ⅰ and Sal Ⅰ sites of E.coli yeast shuttle plasmid pCLWA2 containing an α Factor promotor leader system. The recombinant plasmid, which contains cecropin AD gene, is named pCAD. It was transferred into yeast host AB103. The transformant was cultured in the liquid SD media (Leu - ) for 48 h at 30 ℃. The expressed cecropin AD that was primary purified from the media showed antibacterial activity by tests of antibacterial PAGE and agar diffussion assay.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1998年第4期33-37,共5页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省自然科学基金
关键词
抗菌肽
表达
酵母
cecropin
expression
yeast
