摘要
目的:研究HPCE法同时测定通塞脉微丸中甘草苷、甘草素和阿魏酸的含量。方法:以葛根素为内标,40mmol·L-1四氢硼钠(pH9.18)-甲醇(90∶10)为运行缓冲液。采用未涂层弹性石英毛细管柱(75μm×50cm,有效长度42.5cm),分离电压20kV;进样压力5kPa,进样1.5s;毛细管温度:25℃;二极管阵列检测,测定波长λs=320nm,λR=380nm。结果:在上述条件下,通塞脉微丸中甘草苷、甘草素和阿魏酸在25min内得到很好的分离,加样回收率分别为99.3%,98.7%,100.2%;RSD分别为3.2%,2.6%和1.7%。结论:本方法具有良好的精密度和回收率,可作为通塞脉微丸质量控制的方法。
Objective：To develop an HPCE method for the determination of liquiritin,liquiritigenin and ferulic acid in Tongsaimai pellets.Methods：Puerarin was used as an internal standard.The buffer was composed of 40 mmol·L^-1borax （pH 9.18）-methanol （90∶10）.CE was performed on a 75 μm×50 cm （effective length was 42.5 cm）fused silica capillary.Separation voltage was 20 kV,sampling pressure was 5 kPa,sampling time was 1.5 s and the temperature was maintained at 25 ℃.Results：Liquiritin,liquiritigenin and ferulic acid were successfully separated within 25 min,the recoveries of them were 99.3%,98.7% and 100.2% and RSDs were 3.2%,2.6% and 1.7%,respectively.Conclusion：This method is convenient,rapid and accurate.It can be used for the determination of liquiritin,liquiritigenin and ferulic acid in Tongsaimai pellets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第4期599-601,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家863重大研究计划项目(2003AA2Z3247)
江苏省科技厅科技攻关重大项目"通塞脉片复方活性部位群及其药效学研究"(BK2001209)
