摘要
目的 银杏内酯提取物中有关微量成分的鉴别。方法 采用LC DAD ESI MS联用技术。结果 鉴别了已知的银杏内酯A ,B ,C ,J,M以及白果内酯 ,并发现了 3个微量未知化合物。结论 根据UV ,IR ,MS和NMR光谱数据 ,鉴定了其中两个新化合物的结构 ,分别为 1,10 二羟基 3,14 二去氢银杏内酯和 10 羟基 3,14 二去氢银杏内酯 ,并命名为GK和GL。
AIM To identify the trace constituents in the total ginkgolide from leaves of Ginkgo biloba . METHODS The extract was analyzed by using LC/DAD/ESI/MS combination technology, both UV and mass spectrums of each compound were recorded simultaneously. UV, IR, MS and NMR spectra were employed for structural identification. RESULTS With higher sensitivity of the MS detector, three trace constituents were found as well as the known compounds ginkgolide A (GA), ginkgolide B (GB), ginkgolide C (GC) and bilobalide (BD). CONCLUSION Two compounds ( t R 57 8 and 56 7 min) were identified as 1,10 dihydroxy 3,14 didehydroginkgolide and 10 hydroxy 3,14 didehydroginkgolide, named as ginkgolide K and ginkgolide L, respectively, according to the characteristics of their UV, MS, IR and NMR spectra. It is the first time of finding the ginkgolide compounds containing double bond.
出处
《药学学报》
CAS
CSCD
北大核心
2001年第8期608-608,共1页
Acta Pharmaceutica Sinica
基金
国家新药基金资助项目(93 -6 7-N -4 5 )
江苏省"三药"重大科技攻关项目(BG2 0 0 0 0 16 )
