摘要
本实验以牛骨为原料,提供了一种经过粉碎、脱脂、脱钙、酶解、盐析、纯化、冷冻干燥等步骤来制备天然牛骨胶原的方法。并采用SDS-PAGE凝胶电泳、紫外吸收光谱、红外吸收光谱、氨基酸含量分析方法,对其进行了结构表征。结果表明,纯化的天然牛骨胶原的纯度达到99%,属于胶原I型([α1(I)]2α2(I)),且完整地保持了胶原的三螺旋结构,两条肽链的相对分子量为α1=123kD,α2=112kD。
In this study, high-purity bone collagen was prepared from bovine bone via smashing, degreasing, decalcifying, hydrolyzing with enzyme, salting out, purifying and freeze-drying, and then its characterization was analyzed by SDS-PAGE electrophoresis, ultraviolet absorption spectrum, infrared absorption spectrum and amino acid component analysis. The results showed that the purity of the collagen product reaches 99%; it belongs to type I collagen （[ α1（I）]2 α2（I）） with integrated three helical structure, and the related molecular weights of its peptide chains α1 and α2 are 123 kDa and 112 kDa, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第7期29-32,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD05A16)
关键词
牛骨
胶原
提纯
结构表征
bovine bone
collagen
purification
characterization
