摘要
在室内培养实验中观测了赤潮生物海洋原甲藻ＰｒｏｒｏｃｅｎｔｒｕｍｍｉｃａｎｓＥｈｒｅｎｂ对ＮＯ、ＮＨ和甘氨酸的需求。结果表明，硝酸盐的浓度从４０到３００μｍｏｌ／ｄｍ３，氨盐浓度从５０到１５０μｍｏｌ／ｄｍ３，甘氨酸和谷氨酸混合液浓度从４０到８０μｍｏｌ／ｄｍ３皆能较好地维持海洋原甲藻的增殖。磷酸盐起着磷源作用。在ＥＤＴＡ浓度为８０μｍｏｌ／ｄｍ３的培养液中，Ｆｅ３＋的浓度从０，．５到１μｍｏｌ／ｄｍ３，Ｍｎ２＋从０到２０μｍｏｌ／ｄｍ３和Ｃｏ２＋从０．１到０．４μｍｏｌ／ｄｍ３能促使海洋原甲藻的增殖。维生素效应试验表明：维生素Ｂ１是生长促进因子，而维生素Ｂ１２和生物素在光和暗处皆未能促进海洋原甲藻的增殖。无机氮和磷的最小细胞额分别为０．７４×１０－１２ｍｏｌ／细胞和０．０４５×１０－１２ｍｏｌ／细胞，其中磷比氮更能限制海洋原甲藻的增殖。
In the laboratory culture experiments it was abserved that the growth of the red tide organism Prorocentrum micans Ehrenb required nitrate, ammonium, and amino acids. The result showed that the appropriate medium concentration for keeping the growth of P. micans should be: nitrate from 40 to 300μmol/dm3, ammonium 50￣150μmol/dm3, and amino acids 40￣80μmol/dm3. Phosphate is the main phosphorous source in ocean. In the medium with 80μmol/dm3 EDTA, Fe3+ from 0. 5 to 1μmol/dm3, Mn2+ from 0 to 20. 0μmol/dm3 and Co2+from 0. 1 to 0. 4μmol/dm3 could improve the growth of Prorerentrum micans, respectively.Under three vitamins test, only Vit-B1 served as a growth factor, Vit-B12 and Biotin did not improve the growth both in light or darkness conditions. The minimum cell quotaos (q0) for nitrogen and phosphorus were estimated to be 0. 74× 10-12 mol/cell and 0. 045×10-12 mol/cell, of which phosphorus can limite the growth of Prorocentrum micans more than nitrogen.
出处
《东海海洋》
1996年第3期33-38,共6页
Donghai Marine Science
基金
浙江省自然科学基金
关键词
氮
磷
维生素
甲藻
增殖效应
红潮
nitrogen, phosphorus, vitamin, trace metal, red tide, Prorocentrum micans,growth
