摘要
对23个北冬虫夏草菌株发酵液中虫草素的含量进行了测定，发现发酵液中虫草素含量最高的菌株为21号；对该菌株的发酵条件进行优化，获得最适培养基配方为：蔗糖40g／L，蛋白胨15g／L，MgSO4·7H2O0．5g／L，KH2PO40．5g／L，K2HPO4·3H2O 0．5g／L，pH自然；在此培养基中添加0．5g／L腺嘌呤进行培养，可使发酵液中虫草素含量提高约30％，同时腺嘌呤的添加可以加快虫草素的合成。培养方式的考察结果表明：纯静置培养有利于虫草素的产生和积累，静置培养28d，所得发酵液中虫草素含量达2．05g／L，比优化前提高了2倍。
The fermented broths of 23 kinds of Cordyceps militaris strains were compared in terms of cordycepin content and it was found that Strain 21＇s fermented broth was the highest in cordycepin content. Optimization of the fermentation conditions for Strain 21 showed that the optimal medium was 40 g/L sucrose, 15 g/L peptone,0.5 g/L MgSO4-7H2O,0.5 g/L KH2PO4 and 0.5 g/L K2HPO4- 3H20,and its pH value was natural. Culture with this medium to which 0.5 g/L adenine was added could make the cordycepin content of the fermented broth increase by 30%. It was proved that the added adenine could accelerate the synthesis of cordycepin. The investigation of the culture conditions showed that static culture was beneficial to the synthesis and accumulation of cordycepin, and 28 d static culture could gain 2.05 g/L cordycepin in the fermented broth which was two times higher than that before optimization.
作者
刘艳芳
唐庆九
顾俊杰
张劲松
杨焱
冯娜
周帅
魏东芝
LIU Yan-fang ,TANG Qing-jiu ,GU Jun-jie,ZHANG Jing-song, YANG Yan, FENG Na＇, ZHOU Shuai, WEI Dong-zhi（1 National Engineering Research Center of Edible Fungi ;Key Laboratory of Applied Mycological Resources and Utilization ,Ministry of Agriculture ;Shanghai Key Laboratory of Agricultural Genetics and Breeding ; Institute of Edible Fungi, SAAS, Shanghai 201106, China ;2 State Key Laboratory of Bioreactor Engineering ,Luhua Institute of Biotechnology , East China University of Science and Technology, Shanghai 200237, China）
出处
《上海农业学报》
CSCD
北大核心
2010年第3期 26-30,共5页
Acta Agriculturae Shanghai
基金
上海市农委重点攻关项目[沪农科攻字（2009）第2-2号）]资助
关键词
北冬虫夏草
虫草素
深层发酵
培养方式
Cordyceps rnili taris
Cordycepin
Submerged fermentation Culture conditions
