摘要
研究了11种化学农药对棉花黄萎病菌和生防菌的选择毒性。对棉花黄萎病菌CV-1的毒力测定结果表明,浓度100μg/g具明显抑制效果的有多菌灵、苯菌灵、甲基硫菌灵和粉锈宁;5000μg/g下具抑制作用的有菌核净和五氯硝基苯。苯并咪唑类杀菌剂对绿色木霉LTR.2的毒性最高,多菌灵、苯菌灵和甲基硫菌灵的EC50值依次为0.7395、1.6784和0.7370μg/g。多菌灵、苯菌灵和甲基硫菌灵对黄萎病菌的最低抑制浓度分别为0.7、2.3、2.6μg/g;对LTR-2最低抑制浓度分别为1.2、3.4、6.4μg/g。多菌灵对几种生防真菌的毒性选择性比值(TSR)介于1~10之间的有LTR-2、2-②、Tc、Tk7a、GLR,分别为4.6、3.1、4.7、1.2和7.3,为正向调控作用;介于10~100之间的有Sxb,其TSR为27.5,为中度选择性;大于1000的有菌株T9,其TSR值为9145.0,为强烈正向调控作用。苯菌灵的TSR值介于1~10之间的有Sxb、2-②、Tc、Tk7a、GLR,依次为2.8、3.9、5.6、4.5和4.7;介于10~100之间的有LTR-2和1、9,其TSR为13.7和66.5;没有大于100的生防菌菌株。
Eleven chemical pesticides were used to evaluate their selective toxicity to Verticillium dahliae, a pathogen of cotton wilt, and its bio-control strains. Results showed that carbendazim, benlate, thiophanate-methyl and triadimefon had strong inhibitory effects on V. dahliae CV-1 at 100μg/g, but the concentration of dimethachlone and quintozene to suppress the fungus was higher (5000μg/g). Fungicides with benzimidazolyl had the highest toxicity against Trichoderma viride LTR-2. The EC50 value of carbendazim, benlate and thiophanate-methyl were 0.7395, 1.6784 and 0.7370μg/g, respectively. The minimum inhibitory concentration(MIC) of carbendazim, benlate and thiophanate-methyl for control of V. dahliae were 0.7, 2.3 and 2.6μg/g, and that against T. viride LTR-2 were 1.2, 3.4 and 6.4μg/g, respectively. The toxicity selective ratios (TSR) of carbendazim to LTR-2, 2-②, Tc, Tk7a and GLR, that ranged from 1 to 10, were 4.6, 3.1, 4.7, 1.2 and 7.3 respectively, which had positive regulation. The TSR of Sxb was 27.5, which had moderate positive regulation. The TSR of T9 was 9145.0, which had strong positive regulation. The TSR of benlate to Sxb, 2-②, Tc, Tk7a, GLR, LTR- 2 and T9 were 2.8, 3.9, 5.6, 4.5, 4.7, 13.7 and 66.5 respectively, which had no strong positive regulation.
出处
《中国生物防治》
CSCD
北大核心
2006年第1期49-53,共5页
Chinese Journal of Biological Control
基金
山东省科技攻关项目(2003-02)