摘要
目的应用股骨头骨缺损模型研究骨缺损对其上部邻近关节软骨结构与代谢的变化，以期说明骨坏死对关节软骨的可能影响。方法将18只杂种成年犬一侧股骨头制造骨缺损模型，对侧作为对照。分别于4、12、24周各处死5、5、8只动物取材。取材前行股骨头X线检查及大体标本检查。应用HE染色、天狼星红染色及甲苯胺蓝染色方法行关节软骨组织学检查，应用氨基酸分析仪及分光光度计行关节软骨组织化学定量检测并行统计学分析。结果造模术后12周，可见到骨缺损上部邻近软骨细胞减少，细胞核变小，软骨基质染色变浅。术后24周可见到细胞排列不整齐，呈细胞簇状。紧邻骨缺损开口处软骨浅表层出现裂隙及纤维化改变。潮线模糊、中断，部分切片出现软骨血管形成改变，可见血管突破潮线现象。邻近骨缺损处软骨葡胺聚糖（GAG）减少。结论与骨坏死的结构强度和血运损害有相似之处的模型骨缺损，对邻近软骨的结构与代谢有影响，提示骨坏死塌陷前期可能会有邻近软骨的变化。
Objective To investigate the changes of structure and metabolism of the cartilage of femoral head overlying the bone defect in animal model and analyse the possible effect of the necrotic area of the femoral head on the cartilage overlying there. Methods 18 adult hybrid dogs was applied for bone defect model of femoral head. Of the femoral heads, the left was applied for bone defect model , the right for shameoperation. 5, 5, and 8 animals were sacreficed respectively 4, 12 and 24 weeks after operation and the femoral heads were received for examinations. The X-rays films were taken just before the animals were sacreficed. After the cartilage specimens were decalcified, dehydrated and embedded, sections 6, u thick were made. They were stained with hematoxylin-eosin, sirius-red and toilude blue. The biochemical assay were performed. Results 12 and 24 weeks after operation of bone defect, cartilage histology and biochemistry demonstrated the signs of articular cartilage pathological changes. The arrangement of chondrocytes were disorder. The number of chondrocytes decreased. The cartilage surface lost its smoothness, and these kinds of changes were more severe in the area next to the bone defect. Fibrosis and vasiformation were found. Tidemark had the changes of discontinuation and veil. Glycosaminoglycans decreased. Conclusion The bone defect, whose damaged structure strength and decreased blood supply is similar, had effect on the structure and metabolism of the articular cartilage near the bone defect, and sugest osteonecrosis at the stage of procollapse may affect the cartilage overlying it.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第39期2795-2798,共4页
National Medical Journal of China
基金
国家自然科学基金资助项目（30772194）
卫生部临床学科重点项目[2007-2009,卫规财函（2007）353号]
关键词
股骨头
骨坏死
软骨
组织学
葡胺聚糖
Femoral head
Osteonecrosis
Cartilage
Histology
Glycosaminoglycans
