摘要
对不同产区葛粉保健品中葛根素的含量进行分析，拟建立高效液相色谱法快速测定葛粉保健品中葛根素含量的方法。固定相为Agilent—C18柱（4．6mm×300mm，5μm），流动相为甲醇-水（70＋30，体积比）溶液，流速为1．0mL·min^-1，波长为250nm，进样量10μL。结果表明：葛根素的质量浓度在10．00-100．00mg·L^-1范围内与峰面积呈线性关系，加标回收率为99．0％～99．8％，检出限（3S／N）为1．0mg·L^-1，相对标准偏差分别为0．57％，0．64％，1．71％。
Concents of puerarin in kudzu vine root powder produced in different areas in our country were determined by HPLC. The Agilent-C18 column （4. 6 mm×300 mm, 5 μm） was used as the stationary phase, and a mixfure of methanol and H2O, mixed in the volume ratio of 70 to 30, was used as the mobile phase with a flow-rate of 1.0 mL ·min^-1. Volume taken for sample introduction was 10 μL. UV-detections were made at the wavelength of 250 nm. Linear relationship was obtained in the range of 10. 00 to 100. 00 mg · L^-1 between the values of peak area and concentration of puerarin, with its detection limit （3S/N） of 1.0 mg · L^-1. Test for precision was made at 3 different concentration levels of 1.58, 1.55 and 0. 24 mg · L^-1 for 6 determinations each, giving values of RSD＇s of 0. 57%, 0. 64% and 1.71% respectively. Under the condition of sample analysis, recovery of the method was tested by addition of standard solution of puerarin at 3 different concentrations, and values of recovery （n =6） obtained were in the range of 99. 0% to 99. 8%
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2008年第10期945-946,949,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
高效液相色谱法
葛粉
葛根素
保健品
HPLC
Kudzu vine root powder
Puerarin
Health-care food
