摘要
目的:测定葛根素(Pue)对大鼠背根神经节(DRG)细胞河豚毒素不敏感性(TTXr)钠电流的作用。方法:采用全细胞钳制技术,记录成年Wistar大鼠DRG神经元中TTXr钠电流。结果:Pue在0.01-2mmol·L^(-1)浓度范围内,对TTXr钠电流的抑制率为9.5％-83.2％。该抑制作用为浓度依赖性,可部分洗脱,但非频率依赖性或电压依赖性。Pue不影响失活曲线,但使1/2最大激活电压由-26mV升至-16mV,说明抑制了激活过程。结论:Pue抑制大鼠DRG细胞中TTXr钠电流。
AIM: To test if puerarin (Pue) affects slow sodium current in dorsal root ganglion (DRG) neurons. METHODS' Tetrodotoxin resistant (TTXr) sodium current was recorded with whole cell patch clamp technique on DRG neurons of young adult rats. RESULTS: Pue 0.01 - 2 mmol · L-1 inhibited TTXr sodium current by 9.5 % - 83.2 % . The inhibition was concentration-dependent and partially reversible, but was not use-dependent nor voltage-dependent. Pue did not affect the inactivation but changed the potential for half maximal conductance from - 26 mV to - 16 mV, suggesting the activation process was inhibited. CONCLUSION: Pue moderately inhibits TTXr sodium current of rat DRG neurons.
出处
《中国药理学报》
CSCD
1996年第2期115-118,共4页
Acta Pharmacologica Sinica
关键词
葛根素
钠通道
河豚毒素
脊神经节
脑保护作用
puerarin
sodium channels
tetrodotoxin
spinal ganglia
patch-clamp techniques
