摘要
应用AFLP分子标记,对不同年代和地点采收集的长江中华绒螯蟹天然样本(1998年江苏镇江江段,2005年和2006年江苏南京江段,2004年、2005年及2006年长江口)的遗传变异进行了初步分析。结果发现:六个采集样本在等位基因平均数、有效等位基因数、Ne i’s基因多样性指数、Shannon信息指数等4项遗传多样性指标方面均未出现显著差异(P>0.05),但长江口3个采集样本的这4个指标都略高于长江干流的3个采集样本;AMOVA分析显示,样本间的遗传变异仅为3.89%,而样本内的遗传差异却高达96.11%;UMPGA树分析显示,2004-2006采集样本与1998年采集样本之间有一定的遗传差异。研究结果初步表明,不同年代与地点的长江中华绒螯蟹天然群体的遗传变异没有出现显著分化;同20世纪90年代末的样本相比,本世纪初的样本产生了一定程度的遗传差异,但未达显著水平。
AFLP marker was used to evaluate the current genetic variability of Chinese mitten crab （Eriocheir sinensis） from the Yangtze River. Six samplings of Chinese mitten crab were sampled in different locations of the Yangtze River and different years （Zhenjiang section in 1998, Nanjing section in 2005 and 2006, the Yangtze Rive estuary in 2004, 2005 and 2006 ）. The results indicated that： there were no significant differences in mean allele numbers （ Na）, effective allele numbers （ Ne ）, Net＇ s diversity indexes （ H ） and Shannon information indexes（I） among six samplings（P 〉 0.05）. AMOVA analysis showed that 3.89% of the total variation were among samplings, and 96. 11% were contributed within samplings. UMPGA tree showed some genetic divergence between 2004 -2006 samplings and 1998 samplings. It was preliminarily indicated that there was no significant genetic vafiabihty from wild population of the Chinese mitten crab in different locations of the Yangtze River and different years, and there were some genetic divergences between the samplings in 2004 -2005 and the samplings in 1998, but these genetic divergences had not reached a significant level.
出处
《上海水产大学学报》
CSCD
北大核心
2008年第4期385-389,共5页
Journal of Shanghai Fisheries University
基金
上海市科委重大科技攻关项目(04DZ19306)
上海市农委科技兴农重点攻关项目(沪农科攻字2004第8-7号)
上海市重点学科建设项目(YI101)
关键词
中华绒螯蟹
AFLP
遗传变异
Eriocheir sinensis
amplified fragment length polymorphism （ AFLP）
genetic variability
