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中华绒螯蟹遗传变异的RAPD分析 被引量:48

Genetic variation of the Chinese mittenhanded crab (Eriocheir sinensis) populations detected by RAPD analysis
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摘要 用31个可重复性好的随机引物对中华绒螯蟹(Eriocheirsinensis)的辽河、瓯江和长江种群进行了RAPD分析。121个扩增片段中有27个多态片段。遗传距离指数(D)表明中华绒螯蟹种内遗传变异较低(D=0004~0063)。3个种群中,辽河种群(D=0018)和瓯江种群(D=0025)遗传变异较高,而长江种群遗传变异较低(D=0013);辽河种群和瓯江种群间遗传距离(D=0023)小于它们与长江种群间的遗传距离(D值分别为0028和0032)。提示人类经济活动可能已使这2个种群发生了基因交流。 RAPD was employed to assess the genetic variation among Eriocheir sinensis populations of the Yangtze, Liaohe and Oujiang river systems. 31 random reproducible primers revealed 121 amplified fragments in which 27 were polymorphic. Intraspecific genetic variation was low (D=0.004~0.063). Intrapopulation genetic variation of Liaohe population and Oujiang population were higher than that of Yangtze population while the interpopulation variation between Liaohe and Oujiang populations was lower than that between either of them and Yangtze population. The results suggest that the human economic activities have probably caused gene exchanges between the two populations.
出处 《生物多样性》 CAS CSCD 1998年第3期186-190,共5页 Biodiversity Science
基金 江苏省应用基础研究计划 江苏省水产局资助
关键词 中华绒螯蟹 遗传变异 RAPD Eriocheir sinensis, genetic variation,RAPD
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