摘要
利用复合酶水解蚕蛹蛋白制备小分子多肽,通过正交实验得到水解工艺的优化组合为:木-Fla复合酶,酶用量为0.5%,底物含量为4%(1∶25),酶解pH为7.0,反应温度为60℃,酶解时间为3h,在此条件下水解,小分子多肽得率为32.16%,同时建立了毛细管凝胶电泳测定蚕蛹多肽分子的检测方法。碳粒廓清实验表明:蚕蛹蛋白酶水解液中小分子多肽可增强小鼠网状内皮系统吞噬功能。
The low molecular peptides were prepared from silkworm pupa protein by hydrolysis with compound enzyme. The results of orthogonal test showed that the optimum hydrolysis conditions was ：the blend of proteolytic enzymes was papain and flavourzyme,the ratio of enzyme to substrate （E/S） was 0.5 % （w/w） ,the substrate concentration was 4,0 % （w/v）, the hydrolyzing reaction occurs at 60℃and pH 7.0 for 3h. The yield of low molecular peptides was 32.16 % under the conditions. Carbon clearance test showed the low molecular peptides preparation from silkworm pupa protein could strengthen the ability of macrophage phagocytosis.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第1期225-227,共3页
Science and Technology of Food Industry
关键词
酶水解
蚕蛹蛋白
免疫活性肽
enzyme hydrolysis
silkworm pupa protein
immuno-active peptides
