摘要
核糖核酸（ＲＮＡ）与稀土铽离子在ｐＨ５．０～６．５条件下能形成具有较强荧光的络合物，其激发峰在２８８ｎｍ处，发射峰为４９４ｎｍ及５４５ｎｍ处．ＲＮＡ在０．１～１０ｍｇ／Ｌ范围内与其荧光强度呈线性关系，其检出限为６．０×１０￣（－８）ｍｏｌ／Ｌ．腺苷酸，尿苷酸，胞苷酸对ＲＮＡ的干扰比较小，因此可在其存在下选择性地测定ＲＮＡ．
It has been found that ribonucleic acid (RNA) can form complex with Tb(Ⅲ ) at PH5. 0─6. 5, which then emits strong characteristic fluorescence of Tb (Ⅲ) . The excitation and emission wavelengths are 288 nm, 494 nm and 545 nm, respectivly. A linear relationship has been obtained between the fluorescene intensity and RNA concentration in the range of 0. 1─10 mg/L. The detection limit is 6. 0 × 10￣(-8)mol/L. The proposed method can be used to determine RNA in the presence of adenylic acid,uridylic acid and cytidylic acid.
基金
国家自然科学基金
关键词
RNA
铽离子
荧光反应
Terbium, Fluorimetry, RNA determination
