摘要
超氧化物岐化酶(SOD)由于具有消除氧自由基的功能,在医药、保健品中具有重要应用价值。我国目前SOD产品主要是从牲畜血液中提取,这个方法所用原料有限,SOD质量不稳定。80年代后,美国和日本先后开发了用发酵法生产SOD,大大地降低了生产成本。但由于SOD为胞内蛋白,因而发酵法产生SOD后提取工艺极为复杂,至少需要六步,一般经过细胞破碎、离心、盐析、透析、离子交换层析(2～3次)和凝胶层析等才能达到比活>3000u/mg。由于细胞破碎主要为机械法(如球磨和超声波法),胞内所有蛋白都释放出来,SOD比活只有10～30u/mg。
A new and simple process to purify superoxide dismutase ( SOD) from & cerevisiae was studied. The SOD was released selectively by treatment of cells using isopropanol. The phosphate buffer extract containing the released SOD was concentrated with PAN hollow fiber ultrafiltration. The SOD was purified by a two -step acectone precipitation. The new process was simple and the cost of the process was inexpensive in compare with traditional proceduces. The recovery and the specific activity of SOD was 66% and 3891u /mg, respectively.
出处
《微生物学报》
CAS
CSCD
北大核心
1997年第1期68-71,共4页
Acta Microbiologica Sinica
基金
国家教委回国人员科研资助金的部分资助。
关键词
超氧化物歧化酶
酵母
发酵法
分离
提纯
Superoxide dismutase, Saccharomyces cereusiae, Purification
