摘要
本文研究了球形芽孢杆菌（Bacillussphaericus）C3－41超氧化物歧化酶（SOD）的产生条件和部分特性。当C3－41菌株处于孢子囊中期时为产SOD酶高峰期，在30℃下的平板培养物及培养基起始pH为中性（pH7．0）时产生的SOD酶比活最高，经硫酸铵分级沉淀，DEAE－32离子交换层析和SephadexG-100凝胶过滤提纯了SOD酶。此酶属Mn－SOD，在25～35℃和pH5～9范围内较稳定，但在55℃下10min完全失活。
The production conditions and properties of Superoxide Dismutase (SOD) in Bacillus sphaericus strain C3-41 were reported in this paper. The experiment results showed that SOD production in B. s C3-41 began in the vegetative cells and reached a maxium at the middle phase of sporangia. It was also showed that 30℃ of growing temperatUre and pH7.0 of the primary growth medium were fit conditions for producting highest specific activity.The SOD of C3-41 was purified by salting out with ammonium sulfate, column chromatagraph on EDAE-32 and sephadex G-100. The enzyme was not inhibited by H2O2 or KCN. Therefore SOD of B. s C3-41 belongs to Mn-SOD. The enzyme was stable in the conditions of temperature range (25~35℃) and pH range (5.0~9.0), but lost itS activity at 55℃ for 10 minutes.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第6期322-324,共3页
Microbiology China
基金
中国科学院区系分类特别支持项目
