摘要
从酿造酱油曲精中分离纯化到1株产中性蛋白酶的菌株米曲霉SW-4,采用紫外线诱变筛选的突变株SW-44比出发菌株酶活力提高1.66倍。单因素试验表明,达到产酶高峰期的条件为麸皮与黄豆粉比值4:1,培养时间60h,最适培养温度30℃,培养基加水量10mL。
An Aspergillus oryzae strain named SW-4, which could produce neutral protease, was isolated and purified from soy sauce Qu. A mutant named SW-44 was achieved from SW-4 by UV mutation. The activity of neutral protease produced by SW-44 was 1.66 times higher than that from SW-4. The single factor test indicated that the highest neutral protease activity was obtained when the fermentation was carried out at 30℃ for 60 h when the water addition was 10 mL and the addition ratio of wheat bran and soybean powder was 4：1.
出处
《中国酿造》
CAS
北大核心
2007年第10期13-15,42,共4页
China Brewing
基金
福建省教育厅B类科研项目(闽教办科[2006]33号)
关键词
中性蛋白酶
紫外诱变
产酶条件
neutral protease
ultraviolet mutation
enzyme production condition
