摘要
采用高效液相色谱法(HPLC)快速测定芦丁,其测定色谱条件为:色谱柱:Kromasil C18,(10μm,4.6×250mm);流动相:甲醇∶水∶冰乙酸(体积比为:40∶60∶1),流速:1.0mL/min;检测波长:330nm,柱温:室温20℃。线性范围50-500μg/mL,R2=0.9999。在上述条件下对苦荞叶中所含芦丁进行了含量测定,分析结果表明,此法简单快速、高效灵敏、重现性好。
HPLC was used to determine rutin content rapidly. The optimum chromatograph parameters are as follow： column as Kromasil C18（10μm,4.6mm × 250mm）, mobile phase as methanol-water-acetic acid （40： 60 ： 1, v/v/v）, flow rate of the mobile phase 1.0mL/min, detection wavelength 330nm. colume temp. 20 ℃, and the linear range is around 50 - 500μg/mL. R^2 = 0. 9999. The proposed method shows a higher accuracy and precision.
出处
《粮油食品科技》
2007年第3期57-58,共2页
Science and Technology of Cereals,Oils and Foods
基金
教育部外司留资助项目内容(200555)
