摘要
在真核生物的基因中,mRNA选择性剪接现象十分普遍。mRNA选择性剪接导致一个基因多转录本的产生,被认为是高等生物增加蛋白质多样性的主要机制,且已发现与许多人类疾病密切相关。发现这些转录本的选择性剪接位点、新的外显子和外显子组合,乃至获得这些剪接变异体的完整克隆,对于基因功能的深入研究十分必要。简要介绍了几种在mRNA水平探索选择性剪接的方法。
mRNA alternative splicing events are widespread in eukaryotic genome. Alternative splicing, leading to the generation of multiple transcripts from single genes, is believed to be the major mechanism expanding protein diversity in higher organisms, and also being correlated with many human diseases. Finding alternative splicing sites, new exons and exon combinations of these transcripts, and obtaining the integrated clone of them are necessary for further research of gene function. Only a reference for readers, several methods about exploring alternative splicing on transcriptional level were introduced briefly here.
出处
《生物技术通讯》
CAS
2007年第4期660-662,共3页
Letters in Biotechnology
