摘要
为了筛选和检测绵羊种群中具有较好表现的微卫星多态性,采用Touch-downPCR方法扩增绵羊基因组上的微卫星DNA序列后,经聚丙烯酰胺凝胶电泳分离,采用改良的银染法对微卫星位点进行多态性检测,染色结果与常规银染法相比,该方法具有灵敏度高、背景浅、污染小、条带清晰等突出特点,能有效地控制染色背景,显著提高检测分辨率,整个染色过程所需时间短,具有广泛的推广价值。
In order to selecting and detecting abundant polymorphism of microsatellite in sheep group,microsatellite DNA which is ampled by the touch down polymerase chain reaction (TD-PCR) is detected by an advanced technique for polyacrylamide gel electrophoresis (PAGE)-silver staining. The band is clearly discernible with satisfactory result.The new silver staining is characterized as weak simple steps, high sensitivity,bright background,little pollution, which is valuable for practical apply.
出处
《生物技术》
CAS
CSCD
2005年第3期51-53,共3页
Biotechnology
基金
国家"十五"科技攻关重大项目(No.2001BA901A16)
