摘要
生物芯片主要有两种类型 ,分别以分子微阵列和结构微阵列为基础 ,前者通过原位化学合成和芯片外合成机械点样法构建高密度的探针微阵列 ,主要用于 DNA序列测定、分析基因组突变和多态性位点以及测定基因表达水平和比较同源基因在不同条件下的表达差异等 ;后者通过电子工业中标准的蚀刻工艺 ,在片基上构建各种显微结构微阵列 ,用于样品的分离及分子扩增等 .本文阐述了生物芯片在概念。
There are two types of biochips, one, containing molecular microarrays which are constructed by the in\|situ chemical synthesis and off\|chip synthesis followed by robotic spotting respectively, is used to detect DNA sequences, screen gene mutations and polymorphisms, analysize the gene expression levels and differential expression of homogenous genes under various conditions; the other, containing structure microarrays which are fabricated by the standard etching technology adopted in electronic industries, is used to separate biological samples and amplify bio\|molecules. In this paper, the concepts, fabrications and applications of biochips are reviewed.
出处
《浙江大学学报（理学版）》
CAS
CSCD
2001年第4期443-449,共7页
Journal of Zhejiang University（Science Edition）
