摘要
目的:建立简化的mRNA差异显示技术中特异条带的回收方法。方法:用单个小鼠早期发育的胚胎构建mRNA差异显示技术,用一步法和煮沸法分别从银染的聚丙烯酰胺凝胶中回收差异显示的特异条带,并进行再扩增、回收、克隆及酶切鉴定。结果:两种不同的回收方法经过mRNA差异显示技术程序环节,证明其具有相同的实验效果。结论:应用简化的一步法和煮沸法对单胚构建的mRNA差异显示技术中的特异条带进行回收,具有有效性和可靠性。
Objective:To establish simplified methods to recover the special bands in the technique of mRNA differential display.Methods:Single-mouse embryo based mRNA differential display technique was established, and the improved one-step and boiling methods were used to recover the special bands from the silver staining PAGE respectively. The obtained special bands were then conducted with the process of reamplifying, recovering, cloning and enzyme digesting.Results:After the process of mRNA differential display technique, it was confirmed that these two different recovering methods had the same experimental results.Conclusion:Using simplified one-step and boiling methods to recover the special bands are effective and reliable in single-embryo based mRNA differential display technique.
出处
《生物技术》
CAS
CSCD
2005年第3期43-44,共2页
Biotechnology
基金
国家自然科学基金资助项目("体外培养的羊早期胚胎发育过程中基因表达的研究"
编号:30270162)
关键词
煮沸法
一步法
回收
MRNA差异显示技术
one-step method
boiling method
recovering
mRNA differential display
