摘要
目的:建立茶黄素类的高效薄层色谱(HPTLC)分离方法,应用聚酰胺薄板为分离材料,以不同溶剂,同一溶剂不同比例及不同预饱和时间分别对茶黄素类(TFs)的分离度进行研究,以前7个峰为考察对象,结果发现,展开剂为甲醇∶丙酮∶冰乙酸=8∶5∶3(体积比),预饱和时间为20min时分离效果最佳,Rf值分别为:0.14、0.22、0.28、0.31、0.35、0.4、0.48。结论:该方法是一种快速、有效、简便的TFs检测方法。
Object： The method mainly studies isolating theaflavins by high performance thin liquid chromatography（HPTLC） on the polyamid-thin-layer. Through three-single experiments, considering the former 7 peaks as the object, the results reveal that the solvent system is the best when methanol： acetone：acetic acid glacial equals 8：5：3 and saturate time is 20min and their Rf of the 7 peaks are 0.14,0.22,0.28,0.31,0.35,0.4,0,48 respectively.Conclusion： It has been proved to be a quick and effective method of detectinq theaflavins.
出处
《食品工业科技》
CAS
CSCD
北大核心
2007年第6期210-212,共3页
Science and Technology of Food Industry
基金
湖南省科技攻关资助项目(03JZY3016)。
