摘要
通过采用高效液相色谱(HPLC)、紫外光谱和黄酮颜色特征反应对芹菜提取物主要成分芹菜素进行定性鉴定,同时建立定量测量芹菜素的高效薄层色谱(HPTLC)方法。结果表明:芹菜提取物的主要成分是黄酮醇-芹菜素,与芹菜素标准品具有相同的光谱和色谱性质;采用G60高效硅胶板,以氯仿-甲醇-水(18:2.3:0.35)为展开剂,芹菜素标样与试样有相同的Rf值为0.65;用最小二乘法作线性回归,芹菜素含量与斑点峰面积的标准曲线回归方程:y=15138x+7594.8,R2=0.993,线性范围:0.332～0.996μg/斑点。精密度实验RSD(n=5)=1.33%,平均回收率97.82%。
By high-performance liquid chromatography （HPLC）, UV spectrum and high-performance lamina chromatography （HPTLC） and color characteristic reaction to quantify the flavonoids, the basic components of thick celery extractions were identified as apigenin based on the quantitative analysis method with high-performance lamina chromatography （HPTLC）. The results indicated that the main component of thick celery flavonoids is apigenin which has the same spectrum and chromatogram characteristics with the same Re value（0.65） as the standard sample by the high-performance silica gel board G60 and the outspread solvent （chloroform： methanol： water, 18：2.3：0.35）. The standard curve regress equation is： y=15138x ＋7594.8, R^2=-0.993 with linearity scope： 0.332 -0.996μg per spot. The precision RSD（n=5） is 1.33 %, and the average callback rate is 97.82%.
出处
《食品科学》
CAS
CSCD
北大核心
2008年第4期291-295,共5页
Food Science
基金
湖南省财政资助项目(60171)
