摘要
目的研究甲醛对小鼠睾丸细胞DNA损伤和交联作用及相关基因Bcl-2、Bax蛋白质表达的影响。方法用0.2～20.0mg/kg的甲醛对小鼠进行染毒,采用彗星实验检测小鼠的睾丸细胞DNA损伤及DNA蛋白质交联;进一步应用免疫组化技术结合显微镜图像分析技术检测睾丸细胞的Bcl-2和Bax的表达情况。结果低浓度(0.2mg/kg)的甲醛能引起小鼠睾丸细胞明显的DNA损伤,高浓度(20.0mg/kg)的甲醛引起DNA蛋白质交联。各浓度处理组Bax阳性表达率明显增多(P<0.05),而各浓度处理组Bcl-2的阳性表达率明显低于对照组(P<0.05)。结论甲醛能引起DNA损伤和交联,以及Bcl-2、Bax的表达发生显著性改变。
Objective To observe the damaging effect of formaldehyde on DNA and the expression related genes of Bcl-2 and Bax in mice testis. Methods Mice were exposed to formaldehyde at the doses of 0. 2 - 20.0 mg/kg. Comet test was used to investigate DNA damage and DNA protein crossiag in mice testis. Immunity technology and microscope image analysis technology was used to further explore testis＇ Bcl-2 and Bax expression. Results At the dose of 0. 2 mg/kg formaldehyde caused obvi- ous DNA damage in mice testis while 20 mg/kg formaldehyde could cause DNA protein crossing. Compared with the control, the positive expression rates of Bax in formaldehyde groups increased obviously, while the positive expression rates of Bcl-2 decreased（P〈0.05）. Conclusion It was suggested that formaldehyde could induce DNA damage and DNA eressing. At the same time, the expression of Bax and Bcl-2 could present obvious expression changes.
出处
《中国职业医学》
CAS
北大核心
2007年第2期90-92,共3页
China Occupational Medicine
基金
浙江省温州市科技发展计划项目(Y2003A017)
关键词
DNA蛋白质损伤
甲醛
相关基因蛋白表达
DNA protein relation damage
Formaldehyde
Related gene protein expression
