摘要
目的研究甲醛对V79细胞DNA的交联作用,探讨甲醛的基因毒作用及可能的形成机制。方法中国仓鼠肺细胞(V79)分别暴露于浓度为75,150,300,600,1200μmol/L的甲醛溶液中1,2,4h,以紫外线照射作为标准断裂剂,用单细胞凝胶电泳技术对不同浓度、不同暴露时间甲醛诱导的V79细胞DNA-交联作用进行体外实验。结果在本研究所用的剂量范围内,除75μmol/L组外,其它4个浓度组3个染毒时间点甲醛均可引起显著的V79细胞DNA的交联作用,彗星尾长和彗星细胞率显著低于紫外线照射的对照组(P<0.01),随着甲醛浓度的增加,交联作用加强,彗星尾长存在明显剂量效应关系(P<0.01)。且细胞暴露在甲醛中4,2h;150,300,600μmol/L浓度组彗星尾长和彗星细胞率显著低于暴露于1h的相应浓度组(P<0.01)。结论甲醛是一种DNA交联剂,具有诱导V79细胞DNA交联的作用,并有明显的剂量、时间效应关系。
Objective To explore genotoxic effects and possible mechanism of formaldehyde on the DNA crosslinks.Methods V79 Chinese hamster cells were exposed to formaldehyde(FA) at different concentration of 75,150,300,600,~1?200 ?μmol/L and incubated for 1,2,4h,respectively.UV were used as standard DNA breaking agents,the DNA crosslinks in V79 Chinese hamster cells induced by different concentration of formaldehyde were detected with Single cell gel electrophoresis assay(SCGE,comet assay)in vitro.Results Formaldehyde could cause DNA crosslinks of V79 Chinese hamster cells within the given doses.Except for the 75?μmol/L dose,the crosslinks were increased with the concentration of formaldehyde and the exposed time in vitro.Conclusion Formaldehyde was a DNA crosslinks agent that could cause DNA cross-links in V79 Chinese hamster cells,and the relationship between DNA crosslinks and concentration was a dose and time-dependent manner in vitro assay.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第1期43-45,共3页
Chinese Journal of Public Health
基金
江苏省社会发展基金资助(BS2001050)
关键词
甲醛
V79细胞
DNA-交联
单细胞凝胶电泳
formaldehyde
V79 Chinese hamster cells
DNA crosslinks
single cellgel electrophoresis assay
