摘要
目的观察Rho/Rho激酶信号转导通路关键信号分子RhoA和磷酸化肌球蛋白轻链[p-MLC(Thr18/Ser19)]在大鼠肝纤维化组织中的表达规律。方法HE及Masson三色染色观察肝病理组织学变化;用Western blot检测RhoA、p-MLC(Thr18/Ser19)蛋白的表达;RT-PCR方法检测RhoAmRNA的表达。结果随着肝纤维化的进展,RhoA、p-MLC(Thr18/Ser19)蛋白表达明显增加,RhoAmRNA基因表达也逐渐加强。RhoA和p-MLC(Thr18/Ser19)分别与α-平滑肌肌动蛋白(α-SMA)呈显著正相关。结论Rho/Rho激酶信号转导通路在肝纤维化形成过程中发生变化。
Objective To observe the expressive changes of RhoA and phosphorylated myosin light chain in rat liver tissue undergoing hepatic fibrogenesis. Methods The liver histopathological changes were evaluated by hematoxylin and eosin staining and Masson＇ s trichrome method. Western blot was used to determine the expressions of RhoA and p-MLC（Thr18/Ser19）, and reverse transcription-polymerase chain reaction （RT-PCR） to determine the expression of RhoA mRNA. Results With the development of hepatic fibrogenesis, the protein expressions of RhoA and p-MLC （Thr18/Ser19） and the mRNA expression of RhoA were significantly increased. RhoA and p-MLC （Thr18/Ser19） correlated with α-SMA positively, respectively. Conclusion Rho/ROCK signaling pathways are nesis.
出处
《基础医学与临床》
CSCD
北大核心
2007年第3期275-278,共4页
Basic and Clinical Medicine
基金
河北省科学技术厅资助项目(06276102D-117)
